Also the results with metabolic poisons suggest that physiological ion gradients sustained by an active Na(+), K(+)-pump are essential for the normal uptake of taurine and hypotaurine.Ĭopyright © 1984. Similarly, prolonged, local release of dibutyryl cyclic-AMP from PLGA microspheres, distributed along the walls of chitosan channels, improves the differentiation of NSPCs within the channels. The uptake of both amino acids was affected more by potassium omission in the dBcAMP-treated than in the control cultures. The uptakes were strictly sodium-dependent and also considerably decreased when potassium ions were omitted from incubation medium. The amounts of cyclic AMP and dibutyryl cyclic AMP used in this work are 10-100 times the normal physiological range, but the amounts of these nucleotides transported across mem. Stimulation of testosterone production in isolated rabbit thecal tissue by LH/FSH, dibutyryl cyclic AMP, PGE2alpha, and PGE2 Endocrinology. Incubation of adipocytes in 0.5 mM dibutyryl cyclic AMP plus 0. In the control cells the transport constant and maximal velocity of taurine uptake were greater in 21-day-old than in 16-day-old cultures, while the changes in hypotaurine transport were the opposite. The regulation of avian lipoprotein lipase by dibutyryl cyclic AMP in cultured adipocytes was studied with quantitative and specific methods for the measurements of enzyme catalytic activity, enzyme protein mass, and immunoadsorption of labeled enzyme. Dibutyryl cyclic AMP induces differentiation of human neuroblastoma SH-SY5Y cells into a noradrenergic phenotype - ScienceDirect. In the treated cultures the kinetic parameters of taurine and hypotaurine uptake remained unaltered during maturation. The dBcAMP treatment produced no marked modification of the transport systems. The uptake of both amino acids consisted of one saturable high-affinity component in both control and treated cultures. AMP on LDL binding uptake and degradation was studied in cultured human skin fibroblasts and in rat and human arterial smooth muscle cells. The characteristics of taurine and hypotaurine uptake were studied during astrocyte maturation in cultures grown in normal, serum-containing medium or in serum-free medium in the presence of 0.1 mmol/l dibutyryl cyclic AMP (dBcAMP).
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